For the complex behaviors of vehicle-bicycle conflict at the conflict zones of channelized islands, the capacity of right-turn lane which is calculated by the traditional analytical method is different with the practical condition. In order to solve the problem, a cellular automaton model based on VISSIM microscopic traffic simulation software for vehicle-bicycle conflict at channelized islands was proposed. According to the proposed rules of cellular automaton, the component object model of VISSIM was used for programming to control the velocity variation of right-turn vehicles by setting a series of detectors which were used to simulate cellular. Therefore, the closure effect of right-turn vehicles when they were in conflict with non-motorized vehicles or pedestrians could be simulated by these settings. Meanwhile, the crossing behaviors of non-motorized vehicles or pedestrians were controlled by using the priority rules of VISSIM. The simulation results show that the average relative error between the capacity of right-turn lane by the proposed model and the practical observation value was 5.45%. The experimental results show that the proposed model is better than the traditional analytical methods and can reflect the practical condition of conflict zones of of channelized islands, thus it can provide theoretical basis for the planning, design, traffic management and organization of channelized islands under the condition of mixed traffic flow.